The BD FACSCanto™ II system is specifically designed to support a wide range of basic flow cytometric applications such as immunophenotyping, cell cycle analysis and apoptosis.
The core laboratory offers two BD FACSCanto™ II systems with 3 lasers (405 nm, 488 nm, 633 nm) and an automated sample loading device (40 tube carousel). It allows the use of up to eight fluorochromes. A barcode reader is available as an extra option. The BD FACSCANTO II™ analysers use FACSDIVA software for acquisition and analysis.
Configuration of the BD FACSCanto™ II analysers
The analysers are 3 laser, 10 parameter instruments (405 nm (violet), 488nm (blue), 633 nm (red)) and are equipped with a carousel loader.
The laser, detector and filter configuration are as follows:
|
Laser
|
LP Mirror
|
BP Filter
|
Intended Dye
|
|
488 nm
Optional
|
735 nm
|
780/60 nm
|
PE-Cy7
|
|
655 nm
|
670 nm
|
PerCP or PerCP-Cy5.5
|
|
556 nm
|
585/42 nm
|
PE
|
|
502 nm
|
530/30 nm
|
FITC
|
|
Blank
|
488/10 and pinhole
|
SSC
|
|
610 nm
|
616/23 nm
|
PE-TexasRed
|
|
633 nm
Optional
|
735 nm
|
780/60 nm
|
APC-Cy7
|
|
Blank
|
660/20
|
APC
|
|
685 nm
|
712/21 nm
|
Alexa700
|
|
405 nm
|
502 nm
|
510/50
|
AmCyan
|
|
Blank
|
450/50 nm
|
Pacific Blue
|
Note
Optional: The core laboratory owns these filters, but they are not routinely installed in the cytometer. If a user requires them for an experiment, please email Susanne Heck in advance.
Booking
These analysers are available 24 hours, 7 days a week except during weekly maintenance and repair periods.
Before an investigator can book and use the equipment, they must attend a basic training class in order to obtain log-in credentials to the instruments and online booking calendar.
Book time on BRC CANTO1
Book time on BRC CANTO2
Please note the following:
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The equipment is available in 15 minute time slots.
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No more than 4 hours can be continuously booked during prime time usage (Monday - Friday, 12pm - 8pm).
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No single user can book more than 8 hours of prime time usage per week.
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Members of the BRC's Faculty of Translational Medicine will be given priority access.